Reporter

Part:BBa_K4799618:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtPCR2_EGFP_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
    Illegal SpeI site found at 1572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
    Illegal XhoI site found at 477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Test if the promoter will induce EGFP express after cadmium treatment.



Source

plasmid

References